400 unique liquid combinations made by hand in under 9 minutes (video cut for time).
To achieve this with pipetting would take 1200 steps!
I am working on a novel liquid handling technique to enable high-throughput maniuplation of liquids without the use of pipettes. This technology is currently in use in the Cira Lab, and we are looking forward to sharing more soon!
Using a crafty combination of high speed imaging and infrared thermography, we found that thermocapillary flows are to blame for the persistence of bubbles in volatile liquids. In these flows, subtle differences in temperature across the bubble cap, just 1-2ÂșC, lead to differences in surface tension at the liquid interface. This difference in surface tension draws liquid into the bubble cap from the surrounding area at a fast enough rate, such that excess pools at the bubble apex and drains down the side in a continually moving rivulet.
The above video received a Gallery of Fluid Motion Award at the annual American Physical Society Division of Fluid Dynamics meeting in 2018.
Results of initial field experiments showing reduced biofouling growth in presence of bubble stream.
Underwater timelapse video of biofouling growth under presence of bubble stream.
Variation of biofouling growth related to bubble stream flowrate Predictions of bubble distributions from bubble plume theory overlayed.
Utilizing both field and laboratory experiments, we've considered various phenomena, including bubble size and flowrate, which may allow bubbles to influence biofouling growth. We've found that liquid shear stresses dominate over other interactions, such as capillary scavenging, light scattering, and gas transfer. The bubbles act to generate motion near the solid interface which sets up a high shear stress that correlates with reducec biofouling growth.
To establish a fundamental understanding, we investigated the process of bubbles sticking to glass slides with various surface treatments. We've found that the behavior at early times does not agree with the existing models for these systems, and in fact the processes happen much more rapidly than initially proposed.
Timeseries from high speed video of air bubble de-wetting beneath a glass slide.